ABSTRACT
Zucchini yellow mosaic virus[ZYMV] is potyvirus with a worldwide distribution. The virus is the most prevalent in cucurbits. Samples of cucumber plants [Cucumls sativus L.] that show symptoms of virus infection were collected during the 18-May-2009 growing season from field-grown cucumber in Riyadh Saudi Arabia. Based on DAS-ELISA with a polyclonal antiserum against ZYMV and reverse transcription polymerase chain reaction [RT-PCR] analysis using two primer pairs, the potyvirus was identified as strain of ZYMV. The anti-ZYMV antibodies in dot-blot, confirming the identity of the isolated viral particles as a potyvirus immunogenically related to ZYMV. Nucleotides Sequence analysis confirms that the isolated virus belong to potyvirus and its gen bank is GU808330.1. This is the first report on ZYMV as causal virus infecting cucumber in KSA
Subject(s)
Cucumber Mosaic Virus Satellite/genetics , Metalloendopeptidases/genetics , Polymerase Chain Reaction/statistics & numerical data , Enzyme-Linked Immunosorbent Assay/methodsABSTRACT
Lethal necrosis or systemic stem necrosis followed by death of Nicotiana benthamiana, severe leaf deformations of N. tabacum cv. white burley and blister formations on N. tabacum cv. samsun NN symptoms were induced by experimental inoculations of CMV RNA preparations containing satellite RNA (sat-RNA). Inoculations of RNA preparations without sat-RNA did not induce that severe symptoms on these plants, only late mild mosaic was observed. It is suggested that sat-RNA of CMV isolate has a certain role for enhancing severity of symptoms in tobacco plants. Local and systemic lethal necrosis of N. benthamiana is due to sat-RNA present with genome of CMV isolate. It is the first report of lethal necrosis induced in N. benthamiana by CMV satellite.
Subject(s)
Cucumber Mosaic Virus Satellite/genetics , Cucumovirus/genetics , Necrosis , Plants, Toxic , VirulenceABSTRACT
Virus strains isolated from Ocimum sanctum and Zinnia elegans were identified as cucumber mosaic virus (CMV) strains on the basis of non-persistant aphid transmission, 28 nm particles, 26 kDa coat protein subunits and serological relationships with CMV and chrysanthemum aspermy virus. The strains showed some biological, serological and satellite RNA based differentiation with other CMV strains isolated earlier from chrysanthemum, petunia and tobacco.